DETERMINATION OF A PROCEDURE FOR QUANTIFICATION OF ANTHOCYANS IN SEA BUCKTHORN (HIPPOPHAE RHAMNOIDES) FRUITS

Download full text PDF
DOI: https://doi.org/None
Issue: 
7
Year: 
2015

O.V. Trineeva (1), PhD; Professor A.I. Slivkin (1), PhD; Professor I.A. Samylina (2), PhD; M.A. Kazmina (1) 1 -Voronezh State University; 1, Universtitetskaya Square, Voronezh 394006, Russia 2 -I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 119991

Sea buckthorn (Hippophae rhamnoides L.) fruits are a valuable source of biologically active substances. Anthocyans, the content of which is not standardized, are present in the sea buckthorn fruits. The authors developed and validated a procedure for the spectrophotometric quantification of the sum of anthocyans in the sea buckthorn fruits, calculated with reference to cyanidin-3-O-glucoside. Optimal conditions for the extraction of anthocyans from the sea buckthorn fruits were determined. The impact of a raw material/reagent ratio, the temperature and frequency of extraction and polarity of a reagent, as well as the impact of the optimal time of extraction of anthocyans to yield were studied. The content of anthocyans in the fruits on drying was established to considerably decrease and amount to less than 30% of the fresh raw material. The validation characteristics of the developed procedure were identified, according to which the latter was valid and suitable for standardization.
Keywords: 
sea buckthorn
Hippophae rhamnoides L.
fruits
contents
spectrometry
validation
References: 
  1. Zolotareva A.M. Osnovy resursosberegayushhey tehnologii pererabotki biomassy Hippophae rhamnoides L. Avtoref. dokt. tehn. nauk. Ulan-Ude`, 2004. 32.
  2. Zolotareva A.M., Chirkina T.F., Gonchikova S.D., Karpenko L.D. Himicheskiy sostav oblepihovogo shrota. Izv. vuzov. Pishhevaya tehnologiya, 1994;.1–2: 24–26.
  3. Karpova E.A. Izmenchivost` biohimicheskogo sostava plodov oblepihi krushinovidnoy pri introdukcii v lesostep` Zapadnoy Sibiri. Avtoref. kand. tehn. nauk. Novosibirsk, 1999; 25.
  4. Kurkin V.A., Ryazanova T.K., Kurkina A.V., Egorova A.V. Razrabotka metodiki opredeleniya antocianov v lekarstvennom rastitel`nom syr`e. Farmaciya, 2014; 4: 17–20.
  5. GOST R 53773-2010. Produkciya sokovaya. Metody opredeleniya antocianov.
  6. Sabir S.M., Magsood N., Hayat Imran et al. Elemental and nutritional analysis of Sea Buckthorn (Nippophae rhamnoides ssp. turkestanica) Berries of Pakistani Origin. J. Med. Food, 2005; 8 (4): 518–522.
  7. Andreeva V.Yu., Kalinkina G.I., Kolomiec N.E`., Isaykina N.V. Metodika opredeleniya antocianov v plodah aronii chernoplodnoy. Farmaciya, 2013; 3: 19–21.
  8. Brezhneva T.A., Logvinova E.E., Slivkin A.I., Tarabrina V.N. Spektral`nye harakteristiki antocianovyh soedineniy plodov ryabiny chernoplodnoy. Vestnik VGU. Ser. Himiya, Biologiya. Farmaciya, 2013; 2: 76–179.
  9. Lomova T.S. Novye resheniya v hromatograficheskom i fotometricheskom analize antocianovyh pigmentov iz rastitel`nogo syr`ya. Avtoref. kand. him. nauk. Voronezh, 2007; 23.
  10. Ryazanova T.K. Farmakognosticheskoe issledovanie plodov i pobegov cherniki obyknovennoy. Fundamental`nye issledovaniya, 2013; 8: 1136–1140.
  11. Trineeva O.V., Kaz`mina M.A., Slivkin A.I. Issledovanie spektral`nyh harakteristik antocianovyh soedineniy plodov oblepihi krushinovidnoy. Vestnik VGU. Seriya: Himiya, Biologiya. Farmaciya, 2014; 3: 118–122.