Quantification of polysaccharides in medicinal plant raw materials

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DOI: https://doi.org/10.29296/25419218-2020-08-01
Issue: 
8
Year: 
2020

S.D. Kakhramanova(1, 3), D.O. Bokov(1, 2), I.A. Samylina(1) 1-I.M. Sechenov First Moscow State Medical University (Sechenov University), 8, Trubetskaya St., Build. 2, Moscow 119991, Russian Federation; 2-Federal Testing Center for Nutrition and Biotechnology, 22/14, Ustinsky Passage, Moscow 109240, Russian Federation; 3-Research Center for Examination of Medical Products, Ministry of Health of the Russian Federation, 8, Petrovsky Boulevard, Build. 2, Moscow 127051, Russian Federation

Main assays for the quantification of polysaccharides in medicinal plant raw materials (MPRM) and herbal remedies (HRs) are considered. It should be noted that the pharmacopoeial methods are gravimetry and spectrophotometry, which have a number of disadvantages directly related to their performance and obtained results. However, with the advent of modern instrumental physicochemical methods, such as HPLC with various types of detection and capillary electrophoresis, it has become possible to substantially improve the quality control for MPRM and HRs. It seems very relevant to more widely use the methods in the pharmacopoeial analysis of MPRM and HRs.
Keywords: 
polysaccharides
quantification
medicinal plant raw materials
herbal remedies
References: 
  1. Karpuk V.V. Pharmacognosy. Minsk: BSU, 2011: 340 (in Russian)
  2. Olennikov D.N., Kashchenko N.I. Polysaccharides. Current state of knowledge: an experimental and scientometric investigation. Khimiya rastitel'nogo syr'ya. 2014; 1: 5–26. DOI: 10.14258/jcprm.1401005 (in Russian)
  3. Kurkin V.A. Pharmacognosy. Samara: «Etching», SamGMU. 2016: 371–5 (in Russian)
  4. Fiorito S. et al. Selenylated plant polysaccharides: A survey of their chemical and pharmacological properties. Phytochemistry. 2018; 153: 1–10. DOI: 10.1016/j.phytochem.2018.05.008
  5. Jiao R. et al. The anti-oxidant and antitumor properties of plant polysaccharides. The American J. of Chinese Medicine. 2016; 44 (3): 463–88. DOI: 10.1142/S0192415X16500269
  6. Qu J. et al. Hepatoprotective effect of plant polysaccharides from natural resources: A review of the mechanisms and structure-activity relationship. International J. of Biological Macromolecules. 2020; 161: 24–64. DOI: 10.1016/j.ijbiomac.2020.05.196
  7. The State Pharmacopoeia of The Russian Federation, XIV-ed. [Electronic resource]. Access mode: http://femb.ru/feml (in Russian)
  8. State Register of Medicines. The Russian Federation Ministry of Health [Electronic resource]. Access mode: https://grls.rosminzdrav.ru (in Russian)
  9. European Pharmacopoeia 10.2-th ed. [Electronic resource]. EDQM (European Directorate for the Quality of Medicines and Healthcare). 2019. Access mode: http://online.edqm.eu/entry.htm.
  10. Drozdova I.L. Isolation and chemical study of polysaccharides in the herb Melilotus altissimus Thuill. Vestnik Voronezhskogo Gosudarstvennogo Universiteta. Seriya: Khimiya. Biologiya. Farmatsiya. 2004; 1: 173–5 (in Russian)
  11. Olennikov D.N., Tankhaeva L.M. Method for the quantitative determination of the total content of polysaccharides in flax seeds (Linum usitatissimum L.). Khimiya rastitel'nogo syr'ya. 2007; 4: 85–90 (in Russian)
  12. Olennikov D.N., Tankhaeva L.M. Method for the quantitative determination of the group composition of the carbohydrate complex of plant objects. Khimiya rastitel'nogo syr'ya. 2006; 4: 29–33 (in Russian)
  13. GPM.1.2.3.0019.15 «Determination of sugars by spectrophotometric method». The State Pharmacopoeia оf The Russian Federation, XIV-ed. [Electronic resource]. Access mode: http://femb.ru/feml (in Russian)
  14. Samylina I.А. et al. Determination of sugars by spectrophotometric methods. Farmatsiya. 2009; 4: 3–5 (in Russian)
  15. State Pharmacopoeia of the Republic of Belarus. II ed. Vol.2. Minsk: Center for Expertise and Testing in Health Care, 2016. (in Russian)
  16. State Pharmacopoeia of the Republic of Kazakhstan. Astana: ZhibekZholy, 2015 (in Russian)
  17. Olennikov D.N., Tankhaeva L.M. Development of a technology for obtaining a dry extract of common plantain. Khimiya rastitel'nogo syr'ya. 2006; 1: 49–54 (in Russian)
  18. Olennikov D.N., Tankhaeva L.M. Method for the quantitative determination of the total content of polyfructans in burdock roots (Arctium spp.). Khimiya rastitel'nogo syr'ya. 2010; 1: 115–20 (in Russian)
  19. Tankhaeva L.M., Olennikov D.N. Method for the quantitative determination of the total content of polyfructans in the roots of milk-govan (Taraxacum officinale Wigg.). Khimiya rastitel'nogo syr'ya. 2010; 2: 85–9 (in Russian)
  20. Korzh A.P. et al. Assay of polysaccharides in the sedgecane (Acorus calamus) rhizomes. Farmatsiya. 2011; 4: 24–6 (in Russian)
  21. Nikulin A.V., Tereshchenko G.S., Potanina O.G. Determination of polysaccharidesand free sugars sum by uv-spectrofotometry in Tussilago farfara leaf. Questions of biological, medical and pharmaceutical chemistry. 2016; 19 (2): 3–7 (in Russian)
  22. Rovkina K.I. et al. Development of a technique for the quantitative determination of polysaccharides in birch leaves. Medical Bulletin of Bashkortostan. 2019; 14, 1 (79): 47–50 (in Russian)
  23. Rovkina K.I. et al. Validation of the spectrophotometric method for the quantitative determination of birch polysaccharides. Medical Bulletin of Bashkortostan. 2019; 14, 2 (80): 48–51 (in Russian)
  24. Skalozubova T.A. et. al. Polysaccharides in stinging nettle (Urtica dioica) leaves and infusion. Farmatsiya. 2012; 2: 5–7 (in Russian)
  25. Trineeva O.V., Slivkin A.I. Determination of the sum of polysaccharides and simple sugars in the leaves of stinging nettle. Vestnik Voronezhskogo Gosudarstvennogo Universiteta. Seriya: Khimiya. Biologiya. Farmatsiya. 2017; 1: 164–9 (in Russian)
  26. Avdeeva Е.Yu., Krasnov Е.А., Semenov А.А. The content of polysaccharides in aboveground parts Saussurea controversa DC. Khimiya rastitel'nogo syr'ya. 2015; 3: 43–8. DOI: 10.14258/jcprm.201503583 (in Russian)
  27. Bukhanova U.N., Popov D.M., Selezenev N.G. Procedure for determination of the sum of fructosans and fructose in the «Lorpolifit» herbal tea. Farmatsiya. 2013; 1: 22–4 (in Russian)
  28. Neneleva E.V., Evdokimova O.V. Quantification of the sugar content in cinnamon bark. Prospects for the development of modern medicine. Collection of scientific papers on the basis of the international scientific and practical conference. Voronezh, 2015; 2: 201–3 (in Russian)
  29. Talal A.D. Development of a method for the quantitative determination of polysaccharides in tea used for the urinary system diseases. Medical and pharmaceutical journal «Pulse». 2008; 10 (3): 479 (in Russian)
  30. Trineeva O.V., Kazmina M.A., Slivkin A.I. Determination of the sum of polysaccharides and reducing monosaccharides in the fruits of sea buckthorn in various ways of conservation. Materials of the VI-th International Scientific and Methodological Conference «Pharmaceutical Education-2016», Voronezh State University. Voronezh, 2016: 547–51 (in Russian)
  31. Trineeva O.V., Kazmina M.A., Slivkin A.I. Development and validation of methods for determining the amount of free and related simple sugars in the fruit of sea buckthorn. Razrabotka i registratsiya lekarstvennikh sredstv. 2017; 1: 138–43. (in Russian)
  32. Bubenchikova V.N., MalyutinaA.Yu. Standardization of Achyrophorus maculatus L. raw material by polysaccharide content. «University Science: a look into the future». Materials of the final scientific. conf. employees of KSMU, Central Chernozem scientific. center of RAMS and branch of RANS, dedicated to the 78-th anniversary of Kursk State Medical University, Kursk, 7 Feb 2013. Kursk, 2013; 2: 39–40 (in Russian)
  33. Boeva S.A., Bubenchikova V.N. Development of a method for quantitative determination of the amount of polysaccharides in the herb of the genus Galinsoga. Scientific notes of the Oryol State University. Series: Natural, technical and medical sciences. 2014; 6: 104–6 (in Russian)
  34. Drozdova I.L., Lupilina T.I. Development of a method for the quantitative determination of water-soluble polysaccharides in hoary alyssum herb. Kursk Scientific and Practical Bulletin «Man and His Health». 2014; 4: 91–6 (in Russian)
  35. Drozdova I.L., Denisova N.N. Development of methods of quantitative determination of water-soluble polysaccharides in Knautia arvensis herb. Scientific statements of the Belgorod State University. Series: Medicine. Pharmacy. 2016; 36, 26 (247): 114–9 (in Russian)
  36. Kazakova V.S. et al. Determination of the quantitative composition of polysaccharide compounds of plants of the genus lungwort. Scientific results of biomedical research. 2016; 2 (4): 73–7. DOI: 10.18413/2313-8955-2016-2-4-73-77 (in Russian)
  37. Koroleva D.S. Development and validation of a method for the quantitative determination of water-soluble polysaccharides in the herb of blackhead officinalis. Vestnik Farmatsii. 2018; 3 (81): 36–42 (in Russian)
  38. Dyakova N.A. et al. Development of a method for the quantitative determination of water-soluble polysaccharides and its validation. Modern pharmacy: problems and development prospects. 2015; 45–8 (in Russian)
  39. Dyakova N.A. et al. Rationalized method for quantitative determination of water-soluble polysaccharides and its validation. Vestnik Voronezhskogo Gosudarstvennogo Universiteta. Seriya: Khimiya. Biologiya. Farmatsiya. 2015; 2: 106–11 (in Russian)
  40. Dyakova N.A. et al. Research on the development and validation of methods for extracting inulin from burdock roots (Arctium lappa L.). Vestnik Voronezhskogo Gosudarstvennogo Universiteta. Seriya: Khimiya. Biologiya. Farmatsiya. 2016; 2-C: 114–8 (in Russian)
  41. Dyakova N.A. Development and validation of a rapid method for the isolation and quantitative determination of water-soluble polysaccharides in burdock leaves (Arctium lappa L.). Khimiya rastitel'nogo syr'ya. 2018; 4: 81–7. DOI: 10.14258/jcprm.2018042195 (in Russian)
  42. Masuko T. et al. Carbohydrate analysis by a phenol–sulfuric acid method in microplate format. Analytical biochemistry. 2005; 339 (1): 69–72.
  43. Zou Y. et al. Response surface methodology for optimization of the ultrasonic extraction of polysaccharides from Codonopsis pilosula Nannf. var. modesta LT Shen. Carbohydrate Polymers. 2011; 84 (1): 503–8.
  44. Wang J. et al. Geographical origin discrimination and polysaccharides quantitative analysis of Radix Codonopsis with micro near-infrared spectrometer engine. J. of Innovative Optical Health Sciences. 2018; 11 (01): 1850004.
  45. Khasanov V.V. et al. Quantification of inulin in dry aqueous extracts from dandelion and burdock roots. New achievements in chemistry and chemical technology of plant raw materials. 2009; 2: 76–8 (in Russian)
  46. Bokov D.O., Khromchenkova E.P., Sokurenko M.S., Vasilev A.V., Bessonov V.V. Development of a technique for the determination of inulin in natural instant chicory after enzymatic hydrolysis by high-performance liquid chromatography. Voprosy pitaniia. 2017; 86 (5): 50–5 (in Russian)
  47. Bokov D.O. et al. Physiochemical Features, Qualitative and Quantitative Analysis, Present Status and Application Prospects of Polysaccharide Gums. International J. of Pharmaceutical Quality Assurance. 2020; 11 (01): 74–82.
  48. Li S.P. et al. Capillary Electrophoresis of Herbal Carbohydrates. Capillary Electromigration Separation Methods. Elsevier. 2018: 373–96. DOI: 10.1016/B978-0-12-809375-7.00018-6
  49. YakubaYu.F., MarkovskyМ.G. The determination of glucose, sucrose and fructose by the method of capillary electrophoresis. Voprosypitaniia (Problems of Nutrition). 2015; 1: 89–94.
  50. Gamini A. et al. Use of Capillary Electrophoresis for Polysaccharide Studies and Applications. In: Schmitt-Kopplin P. (eds) Capillary Electrophoresis. Methods in Molecular Biology, vol 1483. Humana Press, New York, NY, 2016. DOI: 10.1007/978-1-4939-6403-1_17
  51. Stefansson M., Novotny M. Modification of the electrophoretic mobility of neutral and charged polysaccharides. Analytical chemistry. 1994; 66 (20): 3466–71.
  52. Kinoshita M. et al. Determination of molecular mass of acidic polysaccharides by capillary electrophoresis. Biomedical Chromatography. 2002; 16 (2): 141–5.
  53. Taylor D.L. et al. Characterization of gellan gum by capillary electrophoresis. Australian J. of Chemistry. 2012; 65 (8): 1156–64.
  54. Biryulin S.I., Posokina N.E., Trishkaneva M.V. The allocation of carbohydrates from vegetable raw materials and their identification with the use of capillary electrophoresis. Vegetable crops of Russia. 2019; 5: 84–7 (in Russian) DOI: 10.18619/2072-9146-2019-5-84-87
  55. Chen J., Yang F., Guo H., Wu F., Wang X. Optimized hydrolysis and analysis of Radix Asparagi polysaccharide monosaccharide composition by capillary zone electrophoresis. J. of Separation Science. 2015; 38 (13): 2327–31. DOI: 10.1002/jssc.201500120