Antimicrobial activity of essential oil fractions of Siberian fir and Scotch pine

DOI: https://doi.org/10.29296/25419218-2024-01-03
Issue: 
1
Year: 
2024

E.E. Savelyeva(1), A.A. Efremov(1–3), T.V. Rukosueva(1), N.A. Bulgakova(1), A.K. Logunova(1),
1FSBEI НЕ Prof. V.F. Voino-Yasenetsky KrasSMU MOH Russia, Partizan Zheleznyak str., 1, Krasnoyarsk, 660022, Russian Federation;
2-Institute of Space Technologies FRC KSC SB RAS, Akademgorodok str., 50, Krasnoyarsk, 660036, Russian Federation;
3-Siberian Federal University, Svobodny ave., 82А, Krasnoyarsk, 660041, Russian Federation

Introduction. Plants of the pine family, growing mainly in the Northern Hemisphere, are of interest both from an economic and medical point of view. The antimicrobial properties of essential oils of coniferous plants are being actively studied. Objective: to study the antimicrobial activity of the essential oil fractions of Siberian fir and Scotch pine growing in the Krasnoyarsk Territory. Material and methods. Essential oils were isolated by hydrosteam distillation on an all-metal installation with a Clevenger nozzle. The component composition of the essential oil fractions was determined by gas chromatography-mass spectrometry. The disc diffusion method was chosen to detect the antimicrobial activity of essential oil fractions. The following test cultures were used: Staphylococcus aureus (MSSA), Staphylococcus aureus (MRSA), Escherichia coli, Pseudomonas aeruginosa, Candida albicans. Results. In all fractions of the essential oil of Siberian fir and Scotch pine, monoterpenoids predominate, the dominant components are bornylacetate and pinene, respectively. All fractions of both oils showed antimicrobial activity against Staphylococcus aureus (MRSA), Staphylococcus aureus (MSSA) and Candida albicans. Isolated individually dominant compounds bornylacetate (Siberian fir) and pinene (Scots pine) also showed antimicrobial activity. Conclusion. The antimicrobial activity of individual fractions of essential oils of Siberian fir and Scotch pine and the dominant components of the fractions against Staphylococcus aureus (MSSA and MRSA), Escherichia coli, Pseudomonas aeruginosa, Candida albicans was studied. The activity of the fractions is largely due to the dominant components. The fractions enriched in monoterpenoids showed the highest activity. The strain of Staphylococcus aureus (MRSA) turned out to be the most sensitive to the fractions of essential oils.
Keywords: 
essential oil fractions
antimicrobial activity
Siberian fir (Abies sibirica)
Scotch pine (Pinus sylvestris).
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