IMPROVEMENT OF THE CHARACTERISTICS OF IDENTITY OF HELLEBORE (VERTRUM LOBELIANUM BERNH) RHIZOMES AND ROOTS

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DOI: https://doi.org/None
Issue: 
3
Year: 
2016

N.E. Kolomiets, PhD; T.V. Poluektova, PhD; A.Yu. Malyshev Siberian State Medical University; 2, Moskovsky Road, Tomsk 634050

The current pharmacopeia article on hellebore rhizomes and roots needs to be updated. Performed investigations could specify their microscopic signs and develop a procedure for the qualitative determination of steroidal alkaloids in the raw material by thin-layer chromatography. The description of the microscopic signs of hellebore rhizomes and roots is added by valid photos. Chromatographic analysis was carried out using n-hexane-ethanol (1:1). Chromatogram processing yielded a main adsorption zone at Rf 0.33-0.35 with orange-brown fluorescence.
Keywords: 
hellebore
Vertrum lobelianum Bernh.
identity
microscopy
steroidal alkaloids
thin-layer chromatography
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