THE AMINO ACID COMPOSITION OF ALFREDIA CERNUA

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DOI: https://doi.org/None
Issue: 
4
Year: 
2016

I.V. Shilova (1), PhD; N.V. Kuvacheva (2), PhD; Professor I.A. Samylina (3), PhD, Corresponding Member of the Russian Academy of Sciences 1 -E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, 3, Lenin Pr., Tomsk 634028, Russian Federation 2 -Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University; 1, Partisan Zheleznyak St. Krasnoyarsk 600022, Russian Federation 3 -I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 119991, Russian Federation

Background. Alfredia herb extracts have nootropic, antidepressant, anxiolytic, antioxidant, diuretic, and antifungal properties. Amino acids in these extract may contribute a weighty contribution to pharmacological activity. Objective: to investigate the amino acid composition of the above-ground part of the plant Alfredia cernua (L.) Cas.) of the Aster family (Asteraceae). Material and methods. The above-ground Alfredia cernua part stored in the period of flowering to the onset of fruiting in the Ust-Kansky District of the Republic of Altai was the subject of the investigation. The latter was conducted using high performance liquid chromatography, thin-layer chromatography in silica gel, and Kjeldahl method. The content of free and bound amino acids was determined using an A0326V2 amino acid analyzer (Germany). Results and discussion. The total amount of nitrogen-containing compounds (13.75±2.45%) was determined in the above-ground part of Alfredia. The use of the amino acid analyzer could establish the presence of 15 amino acids (2.98±0.13 %), including 7 essential ones (1.02%): valine, histidine, isoleucine, leucine, lysine, threonine, and phenylalanine. Glutamic and aspartic acids, alanine, valine, glycine, lysine, leucine, and proline were present in a predominant concentration. Conclusion. The results suggest that it is promising to use the plant as a source of amino acids involved in the processes of nervous and vascular regulation of different functions of the body, which improve the bioavailability of other biological active substances that form their safe formulations simultaneously potentiating their effect.
Keywords: 
Alfredia cernua (L.) Cass
herb
qualitative composition
content
amino acids
glutamic acid
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